Simultaneous Enantioseparation of Monosaccharides Derivatized with L-Tryptophan by Reversed Phase HPLC
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چکیده
منابع مشابه
A reversed phase HPLC method for the simultaneous determination of all monosaccharides contained in galactosaminoglycan isomers from human aorta proteoglycans.
Monosaccharides obtained by reduction and hydrolysis of galactosaminoglycan isomers, are entirely determined as their perbenzoyl derivatives by reversed phase HPLC, without removal of hexosamines prior to benzoylation. The method is suitable for the analysis of arterial proteoglycan constituent galactosaminoglycans, providing specific, precise and reproducible results. Moreover, synthesis and c...
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Chiral recognition mechanism and enantioseparation conditions of tryptophan enantiomers using ligand exchange chromatography are reported here. The effect of different kinds and concentration of ligands, bivalent copper ion, organic modifier, pH of mobile phase, and temperature on enantioseparation has been evaluated. The results show that the enantioselectivity is strongly affected by the pH a...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2014
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.30.739